3BNT
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site in the Presence of [Co(NH3)6]Cl3 (A1555G mutant, Br-derivative)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91950, 0.91984, 0.91637 |
| Spacegroup name | P 64 |
| Unit cell lengths | 74.865, 74.865, 22.537 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.432 - 2.300 |
| Rwork | 0.217 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.800 |
| Data scaling software | SCALA |
| Phasing software | SOLVE (2.12) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.432 | 37.430 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.072 | 0.052 | 0.263 |
| Total number of observations | 996 | 4921 | |
| Number of reflections | 3374 | ||
| <I/σ(I)> | 5.9 | 9.3 | 2.4 |
| Completeness [%] | 99.9 | 97.2 | 100 |
| Redundancy | 9.9 | 8.3 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, spermine tetrachloride, paromomycin, 2-methyl-2,4-pentanediol, hexammine cobalt chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Sodium cacodylate | ||
| 4 | 1 | 1 | [Co(NH3)6]Cl3 | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | KCl |
