3BNT
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site in the Presence of [Co(NH3)6]Cl3 (A1555G mutant, Br-derivative)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.91950, 0.91984, 0.91637 |
Spacegroup name | P 64 |
Unit cell lengths | 74.865, 74.865, 22.537 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.432 - 2.300 |
Rwork | 0.217 |
R-free | 0.25500 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.800 |
Data scaling software | SCALA |
Phasing software | SOLVE (2.12) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.432 | 37.430 | 2.420 |
High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
Rmerge | 0.072 | 0.052 | 0.263 |
Total number of observations | 996 | 4921 | |
Number of reflections | 3374 | ||
<I/σ(I)> | 5.9 | 9.3 | 2.4 |
Completeness [%] | 99.9 | 97.2 | 100 |
Redundancy | 9.9 | 8.3 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, spermine tetrachloride, paromomycin, 2-methyl-2,4-pentanediol, hexammine cobalt chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | Sodium cacodylate | ||
4 | 1 | 1 | [Co(NH3)6]Cl3 | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | KCl |