3BNS
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site (A1555G mutant, Br-derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.670, 77.120, 56.490 |
| Unit cell angles | 90.00, 115.68, 90.00 |
Refinement procedure
| Resolution | 35.390 - 1.900 |
| Rwork | 0.247 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.818 |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.390 | 35.390 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.031 | 0.018 | 0.378 |
| Total number of observations | 7736 | 8192 | |
| Number of reflections | 21598 | ||
| <I/σ(I)> | 16.2 | 56.4 | 2.8 |
| Completeness [%] | 97.4 | 92.9 | 97.4 |
| Redundancy | 3.76 | 3.57 | 3.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, spermine tetrachloride, tobramycin, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Sodium cacodylate | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | KCl |
