3BNQ
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site in the Presence of SrCl2 (A1555G mutant, Br-derivative)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.92070, 0.92110, 0.91625 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.266, 76.056, 55.912 |
Unit cell angles | 90.00, 117.06, 90.00 |
Refinement procedure
Resolution | 49.791 - 2.000 |
Rwork | 0.229 |
R-free | 0.26900 |
Structure solution method | MAD |
RMSD bond angle | 0.004 |
Data scaling software | SCALA |
Phasing software | SOLVE (2.12) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.791 | 49.790 | 2.110 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.050 | 0.044 | 0.373 |
Total number of observations | 1919 | 5678 | |
Number of reflections | 17519 | ||
<I/σ(I)> | 6.9 | 11.6 | 1.9 |
Completeness [%] | 97.4 | 96.2 | 85.5 |
Redundancy | 3.5 | 3.4 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, spermine tetrachloride, paromomycin, 2-methyl-2,4-pentanediol, SrCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | Sodium cacodylate | ||
4 | 1 | 1 | SrCl2 | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | KCl | ||
7 | 1 | 2 | SrCl2 |