3BNO
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site (Br-derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.140, 45.460, 52.130 |
| Unit cell angles | 71.43, 73.42, 74.05 |
Refinement procedure
| Resolution | 36.880 - 2.350 |
| Rwork | 0.226 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.901 |
| Data scaling software | d*TREK (9.2SSI) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.880 | 36.880 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
| Rmerge | 0.038 | 0.029 | 0.366 |
| Total number of observations | 8691 | 9087 | |
| Number of reflections | 11751 | ||
| <I/σ(I)> | 17.8 | 45.9 | 3.2 |
| Completeness [%] | 97.8 | 92.9 | 97.7 |
| Redundancy | 3.93 | 3.82 | 3.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, spermine tetrachloride, paromomycin, KCl, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Sodium cacodylate | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | KCl |
