3BNN
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 |
Unit cell lengths | 34.610, 44.500, 52.360 |
Unit cell angles | 68.77, 71.30, 67.72 |
Refinement procedure
Resolution | 39.590 - 2.000 |
Rwork | 0.238 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.910 |
Data scaling software | d*TREK (9.2SSI) |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.590 | 39.590 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.031 | 0.019 | 0.369 |
Total number of observations | 4634 | 4292 | |
Number of reflections | 17270 | ||
<I/σ(I)> | 14.6 | 46.7 | 2.5 |
Completeness [%] | 97.0 | 96.2 | 92.6 |
Redundancy | 2.67 | 2.68 | 2.61 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, spermine tetrachloride, paromomycin, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | Sodium cacodylate | ||
4 | 1 | 2 | MPD | ||
5 | 1 | 2 | KCl |