3BNL
Crystal structure of the bacterial ribosomal decoding A site in the presence of [Co(NH3)6]Cl3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91636 |
| Spacegroup name | I 4 |
| Unit cell lengths | 73.599, 73.599, 63.496 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.000 - 2.602 |
| Rwork | 0.220 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.800 | 36.800 | 2.740 |
| High resolution limit [Å] | 2.602 | 8.220 | 2.602 |
| Rmerge | 0.051 | 0.048 | 0.286 |
| Total number of observations | 1116 | 5724 | |
| Number of reflections | 5295 | ||
| <I/σ(I)> | 9.4 | 9.8 | 2.6 |
| Completeness [%] | 99.9 | 98.4 | 100 |
| Redundancy | 7.1 | 6.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, 2-methyl-2,4-pentanediol, spermine tetrachloride, hexammine cobalt chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Sodium cacodylate | ||
| 4 | 1 | 1 | [Co(NH3)6]Cl3 | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | KCl |
