3BNC
Lipoxygenase-1 (Soybean) I553G Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-10 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.201, 92.278, 49.584 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 27.590 - 1.650 |
| R-factor | 0.17026 |
| Rwork | 0.169 |
| R-free | 0.20114 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1f8n |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.518 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.523 |
| Number of reflections | 102487 | |
| <I/σ(I)> | 18.7 | 1.5 |
| Completeness [%] | 99.3 | 92.8 |
| Redundancy | 3.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | PEG 3350, sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
