3BMZ
Violacein biosynthetic enzyme VioE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 141.6 |
| Detector technology | CCD |
| Collection date | 2007-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.527, 82.477, 90.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.310 - 1.210 |
| R-factor | 0.16912 |
| Rwork | 0.168 |
| R-free | 0.19459 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.209 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.250 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Number of reflections | 111465 | |
| <I/σ(I)> | 35.7 | 3.4 |
| Completeness [%] | 90.6 | 54.3 |
| Redundancy | 13.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% (v/v) PEG-400, 20% (w/v) PEG-3350, 0.1 M magnesium chloride, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
