3BM6
AmpC beta-lactamase in complex with a p.carboxyphenylboronic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Detector technology | CCD |
Collection date | 2005-01-12 |
Wavelength(s) | 1.1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.908, 77.180, 97.937 |
Unit cell angles | 90.00, 115.98, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.25800 |
Starting model (for MR) | 1ga9 |
RMSD bond length | 0.006 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.055 |
Number of reflections | 46573 |
<I/σ(I)> | 15.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 295 | potassium phosphate buffer, pH 8.7, vapor diffusion, hanging drop, temperature 295K |