3BLW
Yeast Isocitrate Dehydrogenase with Citrate and AMP Bound in the Regulatory Subunits
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 113.157, 116.346, 163.620 |
| 格子定数 [度] | 98.96, 110.23, 106.63 |
精密化法
| 残基 | 49.383 - 4.300 |
| R因子 | 0.2758 |
| Rwork | 0.274 |
| R-free | 0.31060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3blv |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 4.450 |
| 分解能 [Å] (高) | 4.300 | 4.300 |
| 独立反射数 | 44752 | |
| <I/σ(I)> | 7.9 | 2.4 |
| 完全性 [%] | 90.1 | 66.7 |
| 冗長性 | 3.2 | 2.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 18% polyethylene glycol 3350, 0.2 M potassium sulfate, 10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
