3BLG

STRUCTURAL BASIS OF THE TANFORD TRANSITION OF BOVINE BETA-LACTOGLOBULIN FROM CRYSTAL STRUCTURES AT THREE PH VALUES; PH 6.2

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-05
Detector	RIGAKU
Spacegroup name	P 32 2 1
Unit cell lengths	53.750, 53.750, 111.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.560
R-factor	0.192
Rwork	0.192
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	BLGA IN LATTICE Z AT PH 8.2
RMSD bond length	0.009
RMSD bond angle	26.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.610
High resolution limit [Å]	2.560	2.560
Rmerge	0.066	0.530
Number of reflections	6422
<I/σ(I)>	26.6	2
Completeness [%]	99.1	97.2
Redundancy	3.28	3.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2 *		pH 6.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	reservoir	HEPES	0.01 (M)
3	1	reservoir	ammonium sulfate	2.2-2.8 (M)