3BL5
Crystal structure of QueC from Bacillus subtilis: an enzyme involved in preQ1 biosynthesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 172.057, 75.542, 131.023 |
Unit cell angles | 90.00, 94.73, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.950 |
R-factor | 0.249 |
Rwork | 0.222 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.950 |
Rmerge | 0.060 |
Number of reflections | 35642 |
<I/σ(I)> | 12.3 |
Completeness [%] | 99.6 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 25% PEG 6000, 100 mM NaCl, 100 mM MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |