3BKU
Apo C-terminal Domain of NikR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.350, 59.660, 75.140 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.291 |
| Rwork | 0.288 |
| R-free | 0.33600 |
| Starting model (for MR) | Ni-bound C-terminal domain of NikR (1Q5Y) |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.459 |
| Number of reflections | 17408 | |
| <I/σ(I)> | 17.9 | 1.7 |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 7.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2M Na Tartrate, 20% w/v PEG 3350, pH 8.0, vapor diffusion, hanging drop, temperature 298K |
