3BKT
Copper-bound C-terminal Domain of NikR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.949, 78.442, 81.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.170 - 1.500 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22700 |
| Starting model (for MR) | The tetramer of nickel-bound c-terminal domain of NikR 1q5y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.346 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.059 | 0.263 |
| Number of reflections | 46684 | |
| <I/σ(I)> | 16.9 | 5.3 |
| Completeness [%] | 97.9 | 85.2 |
| Redundancy | 8.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2M Na Tartrate, 20% PEG 3350, pH 8.0, vapor diffusion, hanging drop, temperature 298K |
