3BKQ
Structure of the P368G mutant of PMM/PGM in complex with its substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.604, 74.740, 86.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.200 - 2.050 |
| R-factor | 0.21955 |
| Rwork | 0.218 |
| R-free | 0.24931 |
| Structure solution method | refinement |
| Starting model (for MR) | 1p5g |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.418 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.3.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.132 | 0.491 |
| Number of reflections | 29408 | |
| <I/σ(I)> | 7.2 | 3.1 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 6.69 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | NA,K tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
