3BKJ
Crystal structure of Fab wo2 bound to the n terminal domain of amyloid beta peptide (1-16)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.54180 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.743, 66.632, 115.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.120 - 1.590 |
R-factor | 0.192 |
Rwork | 0.188 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.398 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.120 | 1.634 |
High resolution limit [Å] | 1.590 | 1.593 |
Rmerge | 0.118 | 0.263 |
Number of reflections | 53462 | |
<I/σ(I)> | 14.3 | 2.7 |
Completeness [%] | 99.2 | 92.23 |
Redundancy | 5.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES, 25% (v/v) PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |