3BKH

Crystal structure of the bacteriophage phiKZ lytic transglycosylase, gp144

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9494 0.9794 0.9795 0.9810
Spacegroup name	P 61
Unit cell lengths	63.167, 63.167, 182.019
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.980 - 2.500
R-factor	0.207
Rwork	0.207
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.980	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	14168
<I/σ(I)>	81	15
Completeness [%]	99.9	99.9
Redundancy	11	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.2 M ammonium sulfate, 1.2% (v/v) polyethylene glycol 400, 60 mM HEPES sodium, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K