3BK8
Urate oxidase aza-xanthine complex in cyanide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 2007-05-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.718, 95.105, 103.957 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.2002 |
| Rwork | 0.200 |
| R-free | 0.23010 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2icq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.028 |
| Data reduction software | MOSFLM |
| Phasing software | FFT |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.210 |
| Number of reflections | 50567 | |
| <I/σ(I)> | 22.3 | 4.4 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 4.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 289 | 8.5MG/ML URATE OXIDASE, 0.2 M SODIUM CYANIDE, 50MM TRIS BUFFER, 0.5MG/ML 8-AZAXANTHINE, 15% PEG 8000 , pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
