3BK6
Crystal structure of a core domain of stomatin from Pyrococcus horikoshii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-24 |
Detector | ADSC QUAMTUM 4r |
Wavelength(s) | 0.9791, 0.9796, 0.9900, 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 142.553, 137.150, 58.544 |
Unit cell angles | 90.00, 107.61, 90.00 |
Refinement procedure
Resolution | 19.970 - 3.200 |
R-factor | 0.20446 |
Rwork | 0.198 |
R-free | 0.26234 |
Structure solution method | MAD |
RMSD bond length | 0.022 |
RMSD bond angle | 2.176 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.058 | 0.474 |
Number of reflections | 17735 | |
<I/σ(I)> | 33.5 | 3.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% MPD, 0.2M ammonium sulfate, 0.1M HEPES-NaOH, 6% 1,5-diaminopentane-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |