3BK5
Crystal structure of putative outer membrane lipoprotein-sorting protein domain from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 48.157, 128.874, 41.968 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.960 - 2.000 |
| R-factor | 0.18147 |
| Rwork | 0.179 |
| R-free | 0.22646 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.641 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.161 | 0.493 |
| Number of reflections | 17574 | |
| <I/σ(I)> | 16.8 | 2.1 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 7.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.1M Na acetate, 20% PEG 8000, 0.2M MgCl2, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
