3BJT

Pyruvate kinase M2 is a phosphotyrosine binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 1
Unit cell lengths	74.634, 80.707, 107.597
Unit cell angles	69.82, 77.74, 67.97

Refinement procedure

Resolution	48.320 - 2.500
R-factor	0.211
Rwork	0.211
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	67470
<I/σ(I)>	14.9
Completeness [%]	95.9
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	PEG3350, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K