3BJS
Crystal structure of a member of enolase superfamily from Polaromonas sp. JS666
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-02-21 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9796 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 274.640, 274.640, 274.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.20701 |
Rwork | 0.205 |
R-free | 0.26337 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.126 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.180 | 0.510 |
Number of reflections | 24915 | |
<I/σ(I)> | 3.9 | 1.8 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 7.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 30% PEG 2000 MME, 150mM Potassium bromide pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |