3BJE

Crystal structure of Trypanosoma brucei nucleoside phosphorylase shows uridine phosphorylase activity

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-28
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91724, 0.97910, 0.91160
Spacegroup name	P 1 21 1
Unit cell lengths	63.015, 95.387, 63.483
Unit cell angles	90.00, 105.91, 90.00

Refinement procedure

Resolution	35.380 - 1.440
R-factor	0.156
Rwork	0.155
R-free	0.18400
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.325
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.10)
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.500
High resolution limit [Å]	1.440	1.440
Rmerge	0.079	0.637
Number of reflections	123263
<I/σ(I)>	8.4	1.2
Completeness [%]	95.4	73.4
Redundancy	3.4	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	39% PEG 8000, 0.1 M Tris-HCl pH 8.5, 0.1 M Sodium phosphate monobasic, 10 mM Uridine, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K