3BJB
Crystal structure of a TetR transcriptional regulator from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97899, 0.97918 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.767, 93.767, 314.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.460 - 2.500 |
| R-factor | 0.23403 |
| Rwork | 0.232 |
| R-free | 0.27762 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.460 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.105 | 0.634 |
| Number of reflections | 48913 | |
| <I/σ(I)> | 27.8 | 1.8 |
| Completeness [%] | 98.2 | 85.8 |
| Redundancy | 7.8 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.5M Ammonnium sulfate, 0.1M Bis-Tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
