3BJ9
Crystal structure of the Surrogate Light Chain Variable Domain VpreBJ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-30 |
| Detector | OXFORD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 43.811, 67.176, 99.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.970 - 2.000 |
| R-factor | 0.18338 |
| Rwork | 0.181 |
| R-free | 0.23015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.622 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.730 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.235 |
| Number of reflections | 9712 | |
| <I/σ(I)> | 17.5 | 7.3 |
| Completeness [%] | 99.4 | 92.38 |
| Redundancy | 7.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M Imidazole, 1M Sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
