3BJ7
Spermine/spermidine N1-acetyltransferase from mouse: Crystal structure of a ternary complex reveals solvent-mediated spermine binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CAMD BEAMLINE GCPCC |
Synchrotron site | CAMD |
Beamline | GCPCC |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.38 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.857, 97.191, 105.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.400 - 2.200 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.400 |
High resolution limit [Å] | 2.100 |
Number of reflections | 46525 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |