3BJ6
Crystal structure of MarR family transcription regulator SP03579
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-21 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793, 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.701, 55.778, 125.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.400 - 2.010 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.19800 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.381 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.400 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 26944 | |
| <I/σ(I)> | 7.4 | 5.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 15 % Ethanol, 0.1 M Imidazole pH 8.0, 0.2M MgCl2 pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
