3BJ6
Crystal structure of MarR family transcription regulator SP03579
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-21 |
Detector | SBC-3 |
Wavelength(s) | 0.9793, 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.701, 55.778, 125.765 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.400 - 2.010 |
R-factor | 0.163 |
Rwork | 0.161 |
R-free | 0.19800 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.381 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.400 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 26944 | |
<I/σ(I)> | 7.4 | 5.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 15 % Ethanol, 0.1 M Imidazole pH 8.0, 0.2M MgCl2 pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |