3BIN
Structure of the DAL-1 and TSLC1 (372-383) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03992 |
| Spacegroup name | P 65 |
| Unit cell lengths | 135.000, 135.000, 50.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.23000 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.814 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 23489 | |
| <I/σ(I)> | 18.89 | 2.95 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.65 | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% ETOH, 0.1M TRIS PH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
