3BIL
Crystal structure of a probable LacI family transcriptional regulator from Corynebacterium glutamicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-17 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.209, 54.338, 78.650 |
Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.221 |
Rwork | 0.218 |
R-free | 0.27800 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.698 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.922 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.130 | 0.616 |
Number of reflections | 22200 | |
<I/σ(I)> | 16.6 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 294 | 30% PEG 3350, 150mM Ammonium fluoride, pH 7.0, VAPOR DIFFUSION, temperature 294K |