3BII
Crystal Structure of Activated MPT Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Collection date | 2002-01-01 |
Wavelength(s) | 1.1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.796, 48.735, 74.915 |
Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.500 |
R-factor | 0.192 |
Rwork | 0.189 |
R-free | 0.26100 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.520 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.500 | 4.740 | 2.200 |
Rmerge | 0.096 | 0.069 | 0.308 |
Number of reflections | 9359 | ||
<I/σ(I)> | 13 | ||
Completeness [%] | 87.6 | 99.6 | 36.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1.1 Ammonium sulfate and .1M HEPES pH=7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |