3BIG
Crystal structure of the fructose-1,6-bisphosphatase GlpX from E.coli in complex with inorganic phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-06-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 4 2 2 |
Unit cell lengths | 91.348, 91.348, 86.366 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.860 - 1.850 |
R-factor | 0.19175 |
Rwork | 0.189 |
R-free | 0.23922 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ni9 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.498 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.870 |
High resolution limit [Å] | 1.810 | 1.810 |
Number of reflections | 33731 | |
<I/σ(I)> | 44.8 | 3 |
Completeness [%] | 99.3 | 92.9 |
Redundancy | 12 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 28% PEG 400, 0.2M CaCl2, 0.1M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |