3BHS
Nitrosomonas europaea Rh50 and mechanism of conduction by Rhesus protein family of channels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2007-03-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | H 3 |
| Unit cell lengths | 96.675, 96.675, 137.621 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.060 - 1.990 |
| R-factor | 0.1963 |
| Rwork | 0.196 |
| R-free | 0.23666 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ns1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.487 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 32561 | |
| <I/σ(I)> | 14.7 | 2.4 |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 6.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1:1 microliter drop ratio of (Protein in 0.02M HEPES pH 7.4, 0.1M NaCl, 0.04M B-octyl glucoside, 2mM Dithiothreitol) and (100mM HEPES, 25% PEG 400). Cryoprotected with 10% Glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
