3BHK
Crystal structure of R49K mutant of monomeric sarcosine oxidase crystallized in phosphate as precipitant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.653, 69.655, 73.357 |
| Unit cell angles | 90.00, 93.74, 90.00 |
Refinement procedure
| Resolution | 32.400 - 1.710 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.20800 |
| Structure solution method | Refined directly |
| Starting model (for MR) | 1L9F |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.063 | 0.264 |
| Number of reflections | 74292 | |
| <I/σ(I)> | 14.9 | 2.3 |
| Completeness [%] | 94.1 | 82.2 |
| Redundancy | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 1.8 M Na/K phosphate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
