3BHG
Crystal structure of adenylosuccinate lyase from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 109.216, 109.216, 157.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.710 - 1.900 |
| R-factor | 0.16772 |
| Rwork | 0.166 |
| R-free | 0.19529 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.309 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.571 |
| Number of reflections | 37453 | |
| <I/σ(I)> | 59 | 4.75 |
| Completeness [%] | 99.5 | 98.5 |
| Redundancy | 18.3 | 16.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M Bis-tris, 2.0 M Ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
