3BH6

Crystal structure of the RP2-Arl3 complex bound to GppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-26
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	73.860, 78.490, 98.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 2.600
R-factor	0.22029
Rwork	0.215
R-free	0.27078
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ksg 2bx6
RMSD bond length	0.010
RMSD bond angle	1.293
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.810	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	18118
<I/σ(I)>	16.12	3.75
Completeness [%]	99.6	99.9
Redundancy	5.9	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% PEG 3350, 0.2 M potassium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K