3BH0
ATPase Domain of G40P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2005-12-16 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 |
| Unit cell lengths | 96.779, 96.779, 54.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.2403 |
| Rwork | 0.238 |
| R-free | 0.28510 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.338 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.032 | 0.320 |
| Number of reflections | 12256 | |
| <I/σ(I)> | 43 | 6 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 7.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M sodium citrate (pH 5.6), 8-12% PEG-4000, 0.2 M ammonium acetate, 1 mM ATP-gamma-S, VAPOR DIFFUSION, HANGING DROP, temperature 100K, temperature 291K |
