3BGT
Structural Studies of Acetoacetate Decarboxylase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-28 |
Detector | Quantum-315 |
Wavelength(s) | 0.9793 |
Spacegroup name | H 3 |
Unit cell lengths | 105.447, 105.447, 252.379 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.300 - 2.100 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.24500 |
Structure solution method | SAD |
RMSD bond length | 0.029 |
RMSD bond angle | 1.961 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.300 |
High resolution limit [Å] | 1.990 |
Number of reflections | 133725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.7 | 291 | 0.5M potassium phosphate dibasic/sodium phoshpate monobasic, pH 7.7, hanging drop, temperature 291K |