3BGH
Crystal structure of putative neuraminyllactose-binding hemagglutinin homolog from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.508, 77.508, 177.509 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.450 |
R-factor | 0.238 |
Rwork | 0.236 |
R-free | 0.27800 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.548 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.020 | 2.580 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.104 | 0.798 |
Number of reflections | 20647 | |
<I/σ(I)> | 14.6 | 2.3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.9 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 294 | 100mM Bis-Tris pH 5.5, 25% PEG 3350, 200mM Lithium sulfate, VAPOR DIFFUSION, temperature 294K |