3BGA
Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97900 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 88.335, 138.864, 102.818 |
Unit cell angles | 90.00, 109.73, 90.00 |
Refinement procedure
Resolution | 49.760 - 2.100 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.084 | 0.189 |
Number of reflections | 118156 | |
<I/σ(I)> | 17.1 | 3 |
Completeness [%] | 86.3 | 37 |
Redundancy | 6.1 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M Magnesium chloride, 20% PEG 3350, 0.1M Bis-tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |