3BG1
Architecture of a Coat for the Nuclear Pore Membrane
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 181.367, 216.786, 192.578 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.29400 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.668 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.110 | 0.646 |
Number of reflections | 63607 | |
Completeness [%] | 100.0 | 92.4 |
Redundancy | 11.2 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.7 | 294 | 16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K |