3BG0

Architecture of a Coat for the Nuclear Pore Membrane

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	181.818, 216.575, 100.959
Unit cell angles	90.00, 108.11, 90.00

Refinement procedure

Resolution	20.000 - 3.150
R-factor	0.252
Rwork	0.252
R-free	0.29400
RMSD bond length	0.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.260
High resolution limit [Å]	3.150	3.150
Rmerge		0.746
Number of reflections	55955
Completeness [%]	100.0	77.7
Redundancy	10.8	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.7	294	16 % (w/v) PEG 3,350, 400 mM NaCl, 100 mM Na-K tartrate, and 100 mM BIS-TRIS, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K