3BFW
Crystal structure of truncated FimG (FimGt) in complex with the donor strand peptide of FimF (DSF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-02-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91168 |
| Spacegroup name | P 1 |
| Unit cell lengths | 24.610, 46.740, 48.620 |
| Unit cell angles | 92.82, 90.92, 92.51 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| Rwork | 0.204 |
| R-free | 0.23800 |
| Starting model (for MR) | 3bfq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.890 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 | 0.108 |
| Number of reflections | 19304 | |
| <I/σ(I)> | 8.4 | 5.3 |
| Completeness [%] | 97.2 | 96.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 mM Tris/HCl, pH 8.0, 80 mM NaCl, 27.5% PEG 1500, 20 mM yttrium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
