3BFR
The crystal structure of Sod2 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 92.740, 92.740, 92.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.050 |
| R-factor | 0.19666 |
| Rwork | 0.195 |
| R-free | 0.23357 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kkc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.992 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.550 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.080 | 0.376 |
| Number of reflections | 16963 | |
| <I/σ(I)> | 18.3 | 5.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 289 | 50% MPD, 0.2M NH4H2PO4,0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, temperature 289K |
