3BFO
Crystal structure of Ig-like C2-type 2 domain of the human Mucosa-associated lymphoid tissue lymphoma translocation protein 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.91790 |
Spacegroup name | P 1 |
Unit cell lengths | 39.944, 43.640, 49.595 |
Unit cell angles | 66.50, 90.00, 90.02 |
Refinement procedure
Resolution | 25.000 - 1.150 |
R-factor | 0.17882 |
Rwork | 0.177 |
R-free | 0.21182 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1U2H 2aw2 1cs6 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.830 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Number of reflections | 103829 | |
<I/σ(I)> | 22 | 2.48 |
Completeness [%] | 94.6 | 91.8 |
Redundancy | 5.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1:1 volume ratio of protein solution (50 mg/mL) and reservoir solution (30% PEG MME 550, 0.2 M Ammonium sulfate, 0.1 M Sodium cacodylate pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K |