3BF2
Crystal structure of the A1KSW9_NEIMF protein from Neisseria meningitidis. Northeast Structural Genomics Consortium target MR36a
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97910, 0.97940, 0.96785 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 65.296, 65.296, 92.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.310 - 2.600 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.067 | 0.463 |
| Number of reflections | 11698 | |
| <I/σ(I)> | 36.89 | 6.72 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 15.1 | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 2.5 M Li chloride, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
