3BEZ
Crystal structure of Escherichia coli Signal peptide peptidase (SppA), SeMet crystals
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC |
Wavelength(s) | 0.97925 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.022, 153.040, 100.214 |
Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
Resolution | 46.630 - 2.760 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.25900 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.892 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.900 | 2.900 |
High resolution limit [Å] | 2.760 | 2.760 |
Rmerge | 0.178 | 0.450 |
Number of reflections | 69169 | |
<I/σ(I)> | 15.6 | 3.3 |
Completeness [%] | 97.8 | 78 |
Redundancy | 7.4 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG3350, pH 7.5, vapor diffusion, hanging drop, temperature 291K |