3BEV
11mer Structure of an MHC class I molecule from B21 chickens illustrate promiscuous peptide binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-07 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.306, 72.199, 72.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.100 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ogt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.327 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.143 | 0.650 |
| Number of reflections | 22499 | |
| <I/σ(I)> | 17.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 27% PEG 3350, 0.1M HEPES pH7.0, 0.1M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
