3BEP
Structure of a sliding clamp on DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.959 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.203, 70.080, 73.872 |
| Unit cell angles | 113.28, 92.07, 99.36 |
Refinement procedure
| Resolution | 36.560 - 1.920 |
| Rwork | 0.210 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pol |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.303 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.069 | 0.395 |
| Number of reflections | 53902 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 95.7 | 79.2 |
| Redundancy | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.9 | 298 | 26% PEG 400, 75mM MES (pH 5.9), 75mM CaCl2, 5% glycerol, 0.5% DMSO, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 10 | 1 | 2 | DMSO | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | CaCl2, | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | DMSO | ||
| 6 | 1 | 2 | PEG 400 | ||
| 7 | 1 | 2 | MES | ||
| 8 | 1 | 2 | CaCl2, | ||
| 9 | 1 | 2 | glycerol |
