3BEO
A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.404, 188.165, 61.679 |
| Unit cell angles | 90.00, 112.22, 90.00 |
Refinement procedure
| Resolution | 43.030 - 1.700 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.26200 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.757 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.346 | |
| Number of reflections | 107005 | |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Hanging drop | 8.5 | 277 | PEG 4000-6000, 100mM Tris-HCl, 0.2M Li2SO4, pH 8.5, Hanging drop, temperature 277K |
