3BED
Mannose/sorbose specific IIA subunit of phosphotransferase system from Enterococcus faecalis
Replaces: 2IACExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.059, 37.503, 76.993 |
| Unit cell angles | 90.00, 121.49, 90.00 |
Refinement procedure
| Resolution | 37.500 - 1.450 |
| R-factor | 0.1593 |
| Rwork | 0.157 |
| R-free | 0.19670 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.805 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.117 | 0.351 |
| Number of reflections | 36836 | |
| <I/σ(I)> | 35.3 | 3.18 |
| Completeness [%] | 79.6 | 31.8 |
| Redundancy | 7.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.2 M CALCIUM CHLORIDE, 0.1 M HEPES BUFFER, 30% PEG 4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
